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Polymerase Chain Reaction (PCR)
A reaction that uses the enzyme DNA polymerase to catalyze the formation of more DNA strands from an original one by the execution of repeated cycles of DNA synthesis. Functionally, this is accomplished by heating and melting double-stranded (hydrogen bonded) DNA into single-stranded (nonhydrogen bonded) DNA and producing an oligonucleotide primer complementary to each DNA strand. The primers bind to the DNA and mark it in such a way that the addition of DNA polymerase and deoxynucleoside triphosphates cause a new strand of DNA to form which is complementary to the target section of DNA. The process described previously is repeated (trait, product, etc.) again and again to produce millions of copies (amplicons) of the desired strand of DNA. PCR and its registered trademarks are the property of F. Hoffmann-La Roche & Co. AG, Basel, Switzerland. POLYMERASE CHAIN REACTION (PCR) TECHNIQUE, NESTED PCR, DEOXYRIBONUCLEIC ACID (DNA), DNA PROBE, PROBE, Q-BETA REPLICASE TECHNIQUE, COCLONING (OF MOLECULES), POSITIVE AND NEGATIVE SELECTION (PNS), AMPLICON, NESTED PCR, PRIMER (DNA), CAPILLARY ELECTROPHORESIS |
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The term "POLYMERASE CHAIN REACTION (PCR)" also appears in the definition(s) of the following term(s): Primer (DNA) Amplicon |
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